Coding
Part:BBa_K3468066:Design
Designed by: Yufei Zhou Group: iGEM20_TJUSLS_China (2020-10-20)
PETase K252L
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around A256 and F271 for mutations, the hydrophobic interaction force between the residues was enhanced and the hydrophobic core was created, so K252L was designed.
Source
Ideonalla sakaiensis